rabbit anti human il 33 antibody  (R&D Systems)

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: Effect of obesity and sex on the circulating levels of interleukin (IL)-33 and soluble suppression of tumorigenicity 2 (sST2). Fasting serum concentrations of ( A ) IL-33, ( C ) sST2 and ( E ) IL-33/sST2 in normal-weight (NW-NG) volunteers ( n = 22), patients with obesity with normoglycemia (OB-NG) ( n = 24) and with obesity-associated type 2 diabetes (OB-T2D) ( n = 27). Circulating concentrations of ( B ) IL-33, ( D ) sST2 and ( F ) IL-33/sST2 in males ( n = 26) and females ( n = 47) volunteers. Data are mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests or by unpaired two-tailed Student’s t test as appropriate. * P < 0.05, ** P < 0.01 and *** P < 0.001

Article Snippet: The blocking solution was then removed, and sections were incubated overnight at 4 °C in a humidified chamber with rabbit anti-human IL-33 antibody (R&D Systems) diluted 1:200 in TBS.

Techniques: Two Tailed Test

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: Gene and protein expression levels of IL-33 in visceral adipose tissue (VAT). A Bar graphs show the gene expression levels of IL33 in VAT samples from volunteers with normal weight (NW-NG, n = 8) and people with obesity and normoglycemia (OB-NG, n = 13) and with obesity-associated type 2 diabetes (OB-T2D, n = 16). B Protein expression levels of IL-33 in VAT from volunteers with normal weight (NW-NG, n = 4) and people with obesity and normoglycemia (OB-NG, n = 13) and with obesity-associated type 2 diabetes (OB-T2D, n = 10). C Scatter plots show the correlations between mRNA expression and protein levels of IL-33 as well as their associations with body fat percentage (BF). Correlation coefficients (r) and corresponding P values were calculated using Pearson’s correlation test. D Representative immunostaining for IL-33 in VAT samples from people with OB-NG and with OB-T2D (magnification 200x). E Comparison of gene expression levels of IL33 with IL1A , IL1B , IL4 , IL6 , IL8 , IL10 , IL13 , IL18 and IL32 in VAT obtained from people with obesity. Values are the mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests or by unpaired two-tailed Student’s t test as appropriate. * P < 0.05, and ** P < 0.01

Article Snippet: The blocking solution was then removed, and sections were incubated overnight at 4 °C in a humidified chamber with rabbit anti-human IL-33 antibody (R&D Systems) diluted 1:200 in TBS.

Techniques: Expressing, Gene Expression, Immunostaining, Comparison, Two Tailed Test

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: Gene expression levels of inflammation-related factors in human visceral adipocytes treated with different concentrations of ( A ) IL-33 and ( B ) LPS for 3 h followed by IL-33 for another 24 h. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. ** P < 0.01. ADIPOQ, adiponectin; IL , interleukin; ITLN1 , omentin; LPS , lipopolysaccharide; TNF , tumor necrosis factor α

Article Snippet: The blocking solution was then removed, and sections were incubated overnight at 4 °C in a humidified chamber with rabbit anti-human IL-33 antibody (R&D Systems) diluted 1:200 in TBS.

Techniques: Gene Expression

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: A Effects of adipocyte-conditioned media (ACM) in monocytes-derived macrophages. Bar graphs show the effect of ACM (20 and 40%) from subjects with obesity incubated for 24 h on the transcript levels of IL33 . Impact of IL-33 on the expression levels of inflammation-related factors in monocytes-derived macrophages primed with ( B ) ACM and ( C ) LPS. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. * P < 0.05 and ** P < 0.01. CCL2 , monocyte chemoattractant protein-1; IL , interleukin; LPS , lipopolysaccharide; TNF , tumor necrosis factor-α

Article Snippet: The blocking solution was then removed, and sections were incubated overnight at 4 °C in a humidified chamber with rabbit anti-human IL-33 antibody (R&D Systems) diluted 1:200 in TBS.

Techniques: Derivative Assay, Incubation, Expressing

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: The increased circulating levels of IL-33 in obesity together the decrease in its soluble decoy receptor sST2 may be potentially enhancing IL-33 bioactivity. In addition, gene and protein expression levels of IL-33 in visceral adipose tissue (VAT) are upregulated in obesity in both adipocytes and stromal vascular fraction cells. IL-33 modulates adipocyte function by upregulating both anti-inflammatory mediators ( ADIPOQ , ITLN1 and IL13 ) and pro-inflammatory cytokines ( TNF , IL8 ). Under inflammatory conditions, such as LPS stimulation, IL-33 attenuates excessive pro-inflammatory responses and promotes protective adipokine expression in visceral adipocytes. In THP-1-derived macrophages, adipocyte-derived factors suppress IL33 expression, whereas IL-33 attenuates macrophage pro-inflammatory activation and enhances IL4 expression only in the context of adipocyte-conditioned medium. Together, these findings suggest the dual and context-dependent nature of IL-33 in obesity, acting as a compensatory mediator to maintain tissue homeostasis while potentially contributing to chronic low-grade inflammation when elevated chronically. ADIPOQ, adiponectin; IL , interleukin; ITLN1 , omentin; TNF , tumor necrosis factor α

Article Snippet: The blocking solution was then removed, and sections were incubated overnight at 4 °C in a humidified chamber with rabbit anti-human IL-33 antibody (R&D Systems) diluted 1:200 in TBS.

Techniques: Expressing, Derivative Assay, Activation Assay